环境微生物实验

中国海洋大学本科生课程大纲

一、课程介绍

1.课程描述：

随着各种环境问题的日趋严重，微生物实验技术在环境科学领域得到广泛应用和迅速发展，为人们改造环境和保护环境提供重要的技术手段。本课程以环境科学领域常用的微生物实验技术的教学为主要内容，并以具体的环境问题为依托，使学生掌握专业知识在实践中的应用，提升学生的实践技能。

2.设计思路：

本课程主要学习和掌握环境科学领域常用的微生物实验技术，了解其在环境保护和环境改造过程中的实践应用及其重要作用。课程内容的设置注重实用性和可操作性，分为基础实验部分和综合实验部分。基础实验部分以常用微生物实验技术的学习为重点，并注重实验内容的系统性和连贯性；综合实验部分以具体环境问题为依托，进行微生物实验技术的实际应用训练。

3.课程与其他课程的关系：

并行课程：环境微生物。

本课程在环境微生物实验技术教学的同时进行环境微生物理论知识的直观验证和感性理解，与环境微生物理论课程的教学相辅相成。

二、课程目标

学生通过课程学习后能够掌握环境科学领域常用的微生物实验技术，并能够在实践应用中初步分析、解释或解决具体环境问题。

三、学习要求

为获得最佳的学习效果，希望学生必须做到以下几点：

1. 课前认真完成实验预习。明确实验目的，理解实验原理，了解实验过程，并主动学习教师布置的相关理论知识，在进入实验室之前对实验相关内容有系统的认识和理解。

2. 按时上课，认真听讲，并按照严格的操作规范完成实验过程，注意记录每个实验阶段的现象或结果，积极参与实验问题的分析、讨论或辩论。

3. 课后主动进行实验反思和深度思考，完成实验报告的撰写。另外，为拓展环境微生物实验相关知识的广度和深度，建议同学课下阅读教师布置的相关内容资料。

四、参考教材与主要参考书

1. 高冬梅, 洪波, 李锋民. 环境微生物实验. 青岛：中国海洋大学出版社, 2009。

2. 肖琳，杨柳燕，尹大强，张敏跃. 环境微生物实验技术.北京：中国环境科学出版社，2004。

3. 沈萍，陈向东. 微生物学实验. 北京：高等教学出版社，2007。

4 钱存柔,黄仪秀. 微生物学实验教程. 北京：北京大学出版社，2008。

5. 周德庆. 微生物学实验教程. 北京：高等教育出版社，2006。

6．马放，任南琪，杨基先. 污染控制微生物学实验. 哈尔滨：哈尔滨工业大学出版社，2002。

五、教学进度

六、成绩评定

（一）考核方式D：A.闭卷考试 B.开卷考试 C.论文 D.考查 E.其他

（二）成绩综合评分体系：

附：评分标准

1）实验操作评分标准

2）问题分析评分标准

3）结果报告评分标准

七、学术诚信

学习成果不能造假，如考试作弊、盗取他人学习成果、一份报告用于不同的课程等，均属造假行为。他人的想法、说法和意见如不注明出处按盗用论处。本课程如有发现上述不良行为，将按学校有关规定取消本课程的学习成绩。
八、大纲审核

教学院长：院学术委员会签章：

Syllabus for undergraduate of OUC

Course name：Experiment of Environmental Microbiology

Course time：32

Course teacher：Dongmei Gao

1.Course Overview

Microbiology experimental technology has been widely used and rapidly developed in the field of environmental science with the increasingly serious environmental problems. It plays an important role in transforming the environment and protecting the environment. The main teaching contents of the course are the common microbiology experimental technologies in various environmental issues.Thecoursewillenablestudentsto master the professional skills.

2.Student Learning Outcomes

Thecoursewillenablestudentsto master the common microbiology experimental technology in the field of environmental science, and to preliminarily analyze, explain or solve the specific environmental problems.

3.Course Expectations

Students should take the initiative to learn the relevant theoretical knowledge on microbiology after class, and finish the homework and the experiment report on time. At the same time, students need pay attention to the practical application of microbial technology.

4.Assignments

Reading materials：

1) Microbes relating to our life

2)Microorganisms in extreme environments

3)Fluorescent probes and detection techniques on microbial cells

4)Principle and application of fluorescence microscope

5)Principle and application of fluorescence spectrophotometer

6)Principle and application of flow cytometry

7)Microbial treatmenttechnologies of pollutants

8)Environmental resource microorganism and application technologies

5.Books To Purchase

1) Lin Xiao, Liuyan Yang, Daqiang Yin, Minyue Zhang. Experimental Technology of Environmental Microbiology. Beijing: China Environmental Science Press, 2004

2)Ping Shen, Xiangdong Chen. Experiment of Microbiology. Beijing: Higher Education Press, 2007

3)Cunrou Qian, Yixiu Huang. Laboratory Experiments in Microbiology. Beijing: BekingUniversity Press, 2008

4)Deqing Zhou. Laboratory Experiments in Microbiology. Beijing: Higher Education Press, 2006

5)Fang Ma, Nanqi Ren, Jixian Yang. Microbiology Experiment in Pollution Control. Harbin: Harbin Institute of Technology Press, 2002

6.Grading Policy

7.Course Calendar

1)Preparation and sterilization of culture medium

Key points: Blending and sterilization of culture medium; Preparation of test tube slant and culture plate

Before class reading: Summary of culture media; Microbial sterilization.

2)Determination of the total number of bacterial colonies in environmental samples

Key points:Gradient dilution; Plate coating technology; Determination of colonies on the plate

Before class reading:Nutrition and reproduction of microorganisms

3） Pure breed separation of microorganisms in environmental samples and slant inoculation technique

Key points: Microbe isolation by streak inoculation; inoculation on test tube slant

Before class reading: Microbe in soil, water and air

4）Bacterial staining and morphological observation

Key points:Principle and operation technology of Gram stain

Before class reading: Microscopic characteristics of microorganisms

5）Micrometer technology and microbe number counting by microscope

Key points: Micrometer technology; microbe number counting by blood cell counter

Before class reading: Methods of microbe number counting

6） Determination of bacterial growth curve

Key points:Growth law and characteristics of bacteria; methods and theories of growth curve determining

Before class reading:Nutrition, metabolism and population growth of microorganisms

7）Bacillus subtilis immobilized by sodium alginate by embedding method

Key points: Methods and theories of microbial cells immobilization; embedding technology

Before class reading: Microbial cells immobilization technology; resource microorganism in various environment

8）Determination of total coliform in water

Key points: Significance, methods and theories of determining total coliform number

Before class reading:Environmental pollution and characteristic microorganism in polluted environment